華中農業大學園藝林學學院果樹系導師介紹：郭文武

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地址：430070 湖北省武漢市華中農業大學園藝植物生物學教育部重點實驗室

Tel: 027-87281543 (O);

Email: guoww@mail.hzau.edu.cn

郭文武簡介：男，1970年9月生，湖北鄖縣人；博士、教授、博士生導師。1993年7月本科畢業于華中農業大學園藝系；1993-1998年，華中農業大學攻讀碩士、博士學位；1998年6月畢業，獲博士學位并留校任教。1999、2003年，分別晉升為副教授、教授。2001-2003年，美國佛羅里達大學柑橘研究與教育中心博士后；2007年，澳大利亞CSIRO園藝部、澳大利亞國立大學高訪3個月；2009年，日本鹿兒島大學高訪2個月。

人才團隊計劃、科研獎勵和榮譽稱號：入選國家杰出青年科學基金（2011）、教育部長江學者特聘教授（2012）、國家特支計劃（萬人計劃）首批科技創新領軍人才、“園藝植物細胞工程與種質創新”教育部創新團隊和湖北省創新研究群體學術帶頭人（2013）、教育部首批新世紀優秀人才、科技部首批中青年科技創新領軍人才；曾獲湖北省自然科學二等獎（排名第二，2001）、國家科技進步二等獎（排名第三，2007）、第11屆中國青年科技獎（2010）。

國內外學術兼職：《Scientia Horticulturae》共同主編（2014.3—）、咨詢編委（2005.3—2014.2），《Plant Cell Tissue Organ Culture》Associate Editor (2009.10—)、《Tree Genetics Genomes》Associate Editor（2011.12—）。中國柑橘學會理事（2009.10—）、中國園藝學會理事（2013.10—）。

主持科研項目：先后主持國家自然科學基金（青年、面上、國家杰青）、科技部973計劃課題、863計劃課題、教育部項目（創新團隊發展計劃、重大項目培育計劃、霍英東青年教師基金、博士點基金及優先課題）、農業部公益性行業科研專項子項目、瑞典國際科學基金、湖北省自然科學基金（創新群體、重點、杰出青年）、武漢市晨光計劃等。

研究興趣與重點內容：從事柑橘種質創新與遺傳改良研究。針對我國柑橘在品種、品質等方面存在的問題，以及常規育種中遇到的珠心胚干擾等生殖障礙，開展細胞工程與遺傳改良研究。旨在提高育種效率、縮短育種周期，培育具有無核、優質、不同熟期等性狀的新品種，充分發揮“細胞工程”這一“傳統且不存在安全性爭議”的生物技術在柑橘遺傳改良中的獨特作用。重點研究內容如下：1）體細胞胚、珠心胚發育的分子機理；2）細胞工程與種質創新：體細胞雜種、胞質雜種創制，三倍體創制，同源多倍體發掘與利用，純系創制，細胞工程新品種選育；3）細胞工程新種質特殊性狀解析與分子育種：胞質雜種雄性不育性狀解析與基因發掘，人工合成多倍體的體細胞互作遺傳、表觀遺傳變異分析，轉基因技術與分子育種。

發表論著：已在J Proteome Res、DNA Research、BMC Genomics、PLoS One、Theor Appl Genet、Planta、Plant Cell Reports、Tree Genetics Genomes、Plant Science、Protoplasma、Genome、Plant Cell Tissue Organ Culture、Plant Breeding、Euphytica、Scientia Horticulturae、中國農業科學、園藝學報等國內外刊物發表第一和通訊作者論文60余篇。部分論著如下（* 通訊作者）：

1.　 解凱東，王惠芹，王曉培，梁武軍，謝宗周，伊華林，鄧秀新，Grosser JW，郭文武*（2013）單胚性二倍體為母本與異源四倍體雜交大規模創制柑橘三倍體。中國農業科學 46：4550-4557

2.　 解凱東，王曉培，王惠芹，梁武軍，謝宗周，郭大勇，伊華林，鄧秀新，Grosser JW，郭文武*（2014）以柑橘多胚性二倍體母本倍性雜交培育三倍體。園藝學報 41：613-620

3.　 梁武軍，解凱東，郭大勇，謝宗周，徐強，伊華林，郭文武*（2014）柑橘10個品種實生后代多倍體的發掘與SSR鑒定。園藝學報 41：409-416

4.　 梁武軍，解凱東，郭大勇，謝宗周，伊華林，郭文武*（2014）10個柑橘砧木類型同源四倍體的發掘與SSR鑒定。果樹學報 31：1-6

5.　Fang YN, Qiu WM, Wang Y, Wu XM, Xu Q, Guo WW* (2014) Identification of differentially expressed microRNAs from a male sterile Ponkan mandarin (Citrus reticulata Blanco) and its fertile wild type by small RNA and degradome sequencing. Tree Genetics Genomes, 10.1007/s11295-014-0780-7

6.　Xu SX, Cai DF, Tan FQ, Fang YN, Xie KD, Grosser JW, Guo WW (2014) Citrus somatic hybrid: an alternative system to studyrapid structural and epigenetic reorganization in allotetraploid genomes. Plant Cell Tissue Organ Culture, DOI: 10.1007/s11240-014-0551-z

7.　Xie KD, Wang XP, Biswas MK, Liang WJ, Xu Q, Grosser JW, Guo WW* (2014) 2n megagametophyte formed via SDR contributes to tetraploidization in polyembryonic ‘Nadorcott’ tangor crossed by citrus allotetraploids. Plant Cell Reports, DOI: 10.1007/s00299-014-1643-2

8.　Zheng BB, Fang YN, Pan ZY, Sun L, Deng XX, Grosser JW, Guo WW* (2014) iTRAQ based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. J Proteome Res 13: 2998-3015

9.　Xiao X, Ma F, Chen CL*, Guo WW* (2014)High efficient transformation of auxin reporter gene into trifoliate orangevia Agrobacterium rhizogenes-mediated co-transformation. Plant Cell Tissue Organ Culture 118: 137-146

10.Xiao SX, Biswas MK, Li MY, Deng XX, Xu Q, Guo WW* (2014) Production and molecular characterization of diploid and tetraploid somatic cybrid plants between male sterile Satsuma mandarin and seedy sweet orange cultivars. Plant Cell Tissue Organ Culture 116: 81-88

11. Ahmad T, Sablok G, Tatarinova T, Xu Q, Deng XX, Guo WW* (2013) Evaluation of codon biology in Citrus and Poncirus trifoliatabasedon genomic features and frame corrected expressed sequence tags. DNA Research 20: 135-150

12.　Liu Z, Ge XX, Wu XM, Kou SJ, Chai LJ, Guo WW* (2013) Selection and validation of suitable reference genes for mRNA qRT-PCR analysis using somatic embryogenic cultures, floral and vegetative tissues in citrus. Plant Cell Tissue Organ Culture 113: 469-481

13.　Guo WW*, Xiao SX, Deng XX (2013) Somatic cybrid production via protoplast fusion for citrus improvement. Scientia Horticulturae 163: 20-26

14.　Qiu WM, Zhu AD, Wang Y, Ge XX, Chai LJ, Deng XX, Guo WW* (2012) Comparative transcript profiling of gene expression between seedless Ponkan mandarin and its seedy wild type during floral organ development by suppression subtractive hybridization and cDNA micro. BMC Genomics 13: 397

15.　Zheng BB, Wu XM, Ge XX, Deng XX, Grosser JW, Guo WW* (2012) Comparative transcript profiling of a male sterile cybrid pummelo and its fertile type revealed altered gene expression related to floral organ development. PLoS ONE 7(8): e43758

16.　Ge XX, Chai LJ, Liu Z, Wu XM, Deng XX, Guo WW* (2012) Transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of Valencia sweet orange by SSH based micro. Planta 236: 1107-1124

17.　Kou SJ, Wu XM, Liu Z, Liu YL, Xu Q, Guo WW* (2012) Selection and validation of suitable reference genes for miRNA expression normalization by quantitative RT-PCR in citrus somatic embryogenic and adult tissues. Plant Cell Reports 31: 2151-2163

18.　Wen L, Tan B, Guo WW* (2012) Estimating transgene copy number in precocious trifoliate orange by TaqMan real-time PCR. Plant Cell Tissue Organ Culture 109: 363–371

19.　Fan J, Liu X, Xu SX, Xu Q, Guo WW* (2011) T-DNA direct repeat and 35S promoter methylation affect transgene expression but do not cause silencing in transgenic sweet orange. Plant Cell Tissue Organ Culture 107: 225-232

20.　Xu SX, Cai XD, Tan B, Li DL, Guo WW* (2011) Effect of ploidy increase on transgene expression: example from Citrus diploid cybrid and allotetraploid somatic hybrid expressing the EGFP gene. Protoplasma 248: 531-540

21.　Amar MH, Biswas MK, Zhang ZW, Guo WW* (2011) Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection. Scientia Horticulturae 128: 220-227

22.　Fu J, Peng ZJ, Cai XD, Guo WW* (2011) Regeneration and molecular characterization of interspecific somatic hybrids between Satsuma mandarin and two seedy sweet oranges for scion improvement. Plant Breeding 130: 287-290

23.　Wu XM, Liu MY, Ge XX, Xu Q, Guo WW* (2011) Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis of Valencia sweet orange. Planta 233: 495-505

24.　Wu XM, Liu MY, Xu Q, Guo WW* (2011) Identification and characterization of microRNAs from citrus expressed sequence tags. Tree Genetics Genomes 7: 117-133

25.　Xu SX, Cai XD, Tan B, Guo WW* (2011) Comparison of expression of three different sub-cellular targeted GFPs in transgenic Valencia sweet orange by confocal laser scanning microscopy. Plant Cell Tissue Organ Culture 104: 199-207

26.　Wang L, Pan ZY, Guo WW* (2010) Proteomic analysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummelo. Plant Cell Tissue Organ Culture 103:165–174

27.　Ge XX, Fan GE, Chai LJ, Guo WW* (2010) Cloning, molecular characterization and expression analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE gene (CitSERK1-like)in Valencia sweet orange. Acta Physiol Plant 32:1197–1207

28.　Duan YX, Fan J, Guo WW* (2010) Regeneration and characterization of transgenic kumquat plants containing the Arabidopsis APETALA1 gene. Plant Cell Tissue Organ Culture 100: 273-281

29.　Cai XD, Duan YX, Fu J, Guo WW* (2010) Production and molecular characterization of two new citrus somatic hybrids for scion improvement. Acta Physiol Plant 32: 215–221

30.　Baig MNR, Yu A, Guo WW*, Deng XX* (2009) Construction, characterization of two Citrus BAC libraries and identification of clones containing Phytoene synthase gene. Genome 52: 484-489

31.　Cai XD, Fu J, Chen CL, Guo WW* (2009) Cybrid / hybrid plants regenerated from somatic fusions between male sterile Satsuma mandarin and seedy tangelos. Scientia Horticulturae 122: 323-327

32.　Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW* (2009) Highly efficient transformation of GFP and KNU genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus. Tree Genetics Genomes 5: 529-537

33.　Guo WW*, Wu RC, Fan GE, Cheng YJ (2008) Analysis of mitochondrial genomes in Citrus interspecific somatic hybrids produced by protoplast fusion. Botanical Studies 49: 295-300

34.　Guo WW*, Wu RC, Cheng YJ, Deng XX (2008) Regeneration and molecular characterisation of two interspecific somatic hybrids for potential citrus rootstock improvement. J Hort Sci Biotech 83: 407-410

35.　Cai XD, Fu J, Deng XX, Guo WW* (2007) Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma and two seedy Citrus cultivars. Plant Cell Tissue Organ Culture 90: 275-283

36.　Duan YX, Liu X, Fan J, Li DL, Wu RC, Guo WW* (2007) Multiple shoot induction from seedling epicotyls and transgenic citrus plant regeneration containing the green fluorescent protein gene. Botanical Studies 48: 165-171

37.　Guo WW*, Wu RC, Cheng YJ, Deng XX (2007) Production and molecular characterization of citrus intergeneric somatic hybrids between red tangerine and citrange. Plant Breeding 126: 72-76

38.　Cai XD, Liu X, Guo WW* (2006) GFP expressionas an indicator of somatic hybrids between transgenic Satsuma mandarin and calamondin at embryoid stage. Plant Cell Tissue Organ Culture 87: 245-253

39.　Guo WW*, Cheng YJ, Chen CL, Deng XX (2006) Molecular analysis revealed autotetraploid, diploid and tetraploid cybrid plants regenerated from an interspecific somatic fusion in Citrus. Scientia Horticulturae 108: 162-166

40.　Guo WW*, Grosser JW* (2005) Somatic hybrid vigor in Citrus: direct evidence from protoplast fusion of an embryogenic callus line with a transgenic mesophyll parent expressing the GFP gene. Plant Science 168: 1541-1545

41.　Guo WW, Duan YX, Olivares-Fuster O, Wu ZC, Arias CR, Burns JK, Grosser JW (2005) Protoplast transformation and regeneration of transgenic Valencia sweet orange plants containing a juice quality related pectin methylesterase gene. Plant Cell Reports 24: 482-486

42.　Guo WW, Prasad D, Cheng YJ, Serrano P, Deng XX, Grosser JW (2004) Targeted cybridization in Citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness. Plant Cell Reports 22: 752-758

43.　Guo WW, Prasad D, Serrano P, Gmitter FG Jr, Grosser JW (2004) Citrus somatic hybridization with potential for direct tetraploid scion cultivar development. J Hort Sci Biotechnol 79: 400-405

44.　Guo WW, Cheng YJ, Deng XX (2002) Regeneration and molecular characterization of intergeneric somatic hybrids between Citrus reticulata and Poncirus trifoliata. Plant Cell Reports 20: 829-834

45.　Guo WW, Deng XX (2001) Wide somatic hybrids of Citrus with its related genera and their potential in genetic improvement. Euphytica 118:175-183

46.　Guo WW, Deng XX, Yi HL (2000) Somatic hybrids between navel orange (Citrus sinensis) and grapefruit (C. paradisi) for seedless triploid breeding. Euphytica 116: 281-285

47.　Guo WW, Zhou CH, Yi HL, Deng XX (2000) Intergeneric somatic hybrid plants between Citrus and Poncirus trifoliata and evaluation of their root rot resistance. Acta Botanica Sinica (currently J Integ Plant Biol) 42: 668-672

48.　Guo WW, Deng XX (1999) Intertribal hexaploid hybrid plants regeneration from electrofusion between diploids of Citrus sinensis and its sexually incompatible relative, Clausena lansium. Theor Appl Genet 98: 581-585

49.　Guo WW, Deng XX (1998) Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Reports 18: 297-300

50.　Guo WW, Deng XX, Shi YZ (1998) Optimization of electrofusion parameters and interspecific somatic hybrid regeneration in citrus. Acta Botanica Sinica(currently J Integ Plant Biol) 40: 417-424

(Updated Sep 2014)